Diversity-generating retroelements for programmable targeted hypermutagenesis.
Rochette P, Lopez-Rodriguez E, Wen DJ, Regnier L, Fan C, Maikova A, Rostain W, Wang L, Nooraddin I, Maire A, Vittot P, Barrabes N, Cerdas-Mejias KM, Bouvier A, Chrysostomou T, Subrini O, Wolff N, Monasson R, Cocco S, Shipman SL, Laurenceau R, Bikard D
Nat Biotechnol. 2026 Apr 16. doi: 10.1038/s41587-026-03078-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250165 | pRL014 | DGRec 2-plasmid system: encodes bRT and avd. |
| 250166 | pRL021 | DGRec 2-plasmid system: encodes CspRecT, MutL* and a dgrRNA cloning site (containing ccdB toxin, to replace with TR). |
| 250169 | pRL038 | DGRec 2 plasmid-system: encodes bRT, avd, and a dgrRNA cloning site (containing ccdB toxin, to replace with TR). Allows dual targeting in combination with pRL021. |
| 250170 | pPR150 | DGRec single plasmid system: encodes bRT, Avd, a dgrRNA cloning site, CspRecT and MutL*. |