Skip to main content
Addgene
Open Menu

pRL021
(Plasmid #250166)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250166 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pRL021
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Plasmid to propagate in a ccdB survival strain. Upon cloning a TR with BsaI, transform into regular DH5alpha to counterselect the parent plasmid.
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Significant fitness cost upon CspRecT prolonged expression.

Please visit https://doi.org/10.1101/2025.03.24.644984 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL021 was a gift from David Bikard (Addgene plasmid # 250166 ; http://n2t.net/addgene:250166 ; RRID:Addgene_250166)

  • For your References section:

    Diversity-generating retroelements for programmable targeted hypermutagenesis. Rochette P, Lopez-Rodriguez E, Wen DJ, Regnier L, Fan C, Maikova A, Rostain W, Wang L, Nooraddin I, Maire A, Vittot P, Barrabes N, Cerdas-Mejias KM, Bouvier A, Chrysostomou T, Subrini O, Wolff N, Monasson R, Cocco S, Shipman SL, Laurenceau R, Bikard D. Nat Biotechnol. 2026 Apr 16. doi: 10.1038/s41587-026-03078-4. 10.1038/s41587-026-03078-4 PubMed 41992038
Related items:
Commonly requested with: