A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesis.
Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl C, Marella N, Buphamalai P, Traversi F, Beres M, Moll HP, Siklos M, Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, Lang K, Huber KVM, Kubicek S
Science. 2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250370 | Lenti-CMV-EGFP-MTHFD1-WPRE | Lentiviral expression of human MTHFD1 with N-terminal EGFP under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250371 | Lenti-CMV-EGFP-MTHFD1 K56R-WPRE | Lentiviral expression of human MTHFD1 carrying K56R mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis. |
| 250372 | Lenti-CMV-EGFP-MTHFD1 K384E-WPRE | Lentiviral expression of human MTHFD1 carrying K384E mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis. |
| 250374 | Lenti-U6-sgRNA_NUDT5_ex4-EF1α-Cas9-NLS-FLAG-GSG-P2A-PuroR-WPRE | Lentiviral expression of sgRNA against human NUDT5 exon 4 under U6 promoter and Cas9-NLS-FLAG-GSG-P2A-PuroR under EF1α core promoter. |
| 250375 | Lenti-CMV-tagBFP-NUDT5-WPRE | Lentiviral expression of human NUDT5 with N-terminal tagBFP under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250376 | Lenti-CMV-tagBFP-NUDT5 E112Q-WPRE | Lentiviral expression of human NUDT5 carrying E112Q mutation with N-terminal tagBFP under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250377 | Lenti-CMV-2xHA-NUDT5-WPRE | Lentiviral expression of human NUDT5 with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250378 | Lenti-CMV-2xHA-NUDT5 P66L-WPRE | Lentiviral expression of human NUDT5 P66L with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250379 | Lenti-CMV-2xHA-NUDT5 C76Y-WPRE | Lentiviral expression of human NUDT5 C76Y with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250380 | Lenti-CMV-2xHA-NUDT5 Y74F-WPRE | Lentiviral expression of human NUDT5 Y74F with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250381 | Lenti-CMV-2xHA-NUDT5 G150V-WPRE | Lentiviral expression of human NUDT5 G150V with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250382 | Lenti-CMV-2xHA-NUDT5 P158I-WPRE | Lentiviral expression of human NUDT5 P158I with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250383 | Lenti-CMV-2xHA-NUDT5 P158T-WPRE | Lentiviral expression of human NUDT5 P158T with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250384 | Lenti-CMV-2xHA-NUDT5 K175M-WPRE | Lentiviral expression of human NUDT5 K175M with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250386 | Lenti-CMV-mCherry-GSG-P2A-NUDT5-WPRE | Lentiviral expression of human NUDT5 with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250387 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 T45A-WPRE | Lentiviral expression of human NUDT5 T45A with N-terminal mCherry seperated by a flexible GSG-linker and P2Aunder CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250388 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 T45D-WPRE | Lentiviral expression of human NUDT5 T45D with N-terminal mCherry seperated by a flexible GSG-linker and P2Aunder CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250389 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A-WPRE | Lentiviral expression of human NUDT5 W28A with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250390 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A W46A-WPRE | Lentiviral expression of human NUDT5 W28A W46A with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250391 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 E112Q-WPRE | Lentiviral expression of human NUDT5 E112Q with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250392 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74E-WPRE | Lentiviral expression of human NUDT5 W28A Y74E with N-terminal mCherry seperated by a flexible GSG-linker and P2A, under CMV promoter as well as a puromycin resistance cassette under PGK promoter. |
| 250393 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 P66L-WPRE | Lentiviral expression of human NUDT5 P66L with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250394 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 C76Y-WPRE | Lentiviral expression of human NUDT5 C76Y with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250395 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A W46A Y74E-WPRE | Lentiviral expression of human NUDT5 W28A W46A Y74E with N-terminal mCherry seperated by a GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250396 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74E E112Q-WPRE | Lentiviral expression of human NUDT5 Y74E E112Q with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250397 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74E-WPRE | Lentiviral expression of human NUDT5 Y74E with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250398 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74F-WPRE | Lentiviral expression of human NUDT5 Y74F with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250399 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74F-WPRE | Lentiviral expression of human NUDT5 W28A Y74F with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250400 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A W46A Y74F-WPRE | Lentiviral expression of human NUDT5 W28A W46A Y74F with N-terminal mCherry seperated by a GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250401 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74F E112Q-WPRE | Lentiviral expression of human NUDT5 Y74F E112Q with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250402 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 G150V-WPRE | Lentiviral expression of human NUDT5 G150V with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250403 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 P158I-WPRE | Lentiviral expression of human NUDT5 P158I with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250404 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 P158T-WPRE | Lentiviral expression of human NUDT5 P158T with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250405 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 K175M-WPRE | Lentiviral expression of human NUDT5 K175M with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter. |
| 250407 | Lenti-EF1α-EGFP-WPRE | Lentiviral expression of EGFP under EF1α core promoter. |
| 250408 | Lenti-EF1α-PPAT-WPRE | Lentiviral expression of PPAT under EF1α core promoter. |