A non-enzymatic role of Nudix hydrolase 5 in repressing purine de novo synthesis.
Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl C, Marella N, Buphamalai P, Traversi F, Beres M, Moll HP, Siklos M, Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, Lang K, Huber KVM, Kubicek S
Science. 2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250377 | Lenti-CMV-2xHA-NUDT5-WPRE | Lentiviral expression of human NUDT5 with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250382 | Lenti-CMV-2xHA-NUDT5 P158I-WPRE | Lentiviral expression of human NUDT5 P158I with N-terminal 2xHA under CMV promoter and puromycin resistance cassette under PGK promoter. |
| 250392 | Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74E-WPRE | Lentiviral expression of human NUDT5 W28A Y74E with N-terminal mCherry seperated by a flexible GSG-linker and P2A, under CMV promoter as well as a puromycin resistance cassette under PGK promoter. |