Functional Mapping and Engineering of the Sec Translocon Unlocked by a Cell-Free System
Meier M, Scholz SA, von Bank L, Levandoski JE, Lückhof M, Schaaf M, Si S, Lieberwirth I, Jung AL, Landfester K, Driessen AJM, Erb TJ
bioRxiv 2025.12.09.688994
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250509 | pTE5601 | Purification of SUMO-dark; inhibitor of 11S; breakage control for translocation/insertion assays |
| 250511 | pTE5603 | Cell-free membrane insertion reporter; proteorhodopsin (UniProt ID: Q9F7P4; C-terminal transmembrane domain deleted); C-terminal pep104 tag |
| 250512 | pTE5605 | Mutant of pSecYEG; impaired in translocation and insertion; cell-free expression of functional SecYEG |
| 250513 | pTE5607 | pSecYEG; cell-free expression of functional SecYEG |
| 250514 | pTE5608 | Cell-free expression of SecA motor protein for translocation through SecYEG |
| 250516 | pTE5627 | Super-active translocator mutant of pSecYEG; cell-free expression of functional SecYEG |
| 250517 | pTE5629 | Cell-free translocation reporter; translocation of a nanobody; N-terminal YncJ signal peptide; C-terminal pep86 tag |
| 250518 | pTE5636 | Cell-free translocation reporter; translocation of a nanobody; N-terminal proOmpA-derived signal peptide with and extended hydrophobic core; C-terminal pep86 tag |
| 250519 | pTE5639 | Superactive inserter mutant of pSecYEG; cell-free expression of functional SecYEG |