Daniel Cifuentes Lab: Cifuentes Lab Plasmids 2025

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
250532	pCS2_N22_HA_dreAdar1	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain on zebrafish Adar1
250533	pCS2_N22_HA_dreAdar1_E488Q	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zerbafish Adar1 (E488Q)
250534	pCS2_EYFP_3xBoxB_Recorder_SV40	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a recorder containing 3 box-b hairpins
250535	pCS2_HA_hsAdar2	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of human Adar2
250537	pCS2_hsAdar2_E488Q_T375G	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of human Adar2 (E488Q, T375G)
250538	pCS2_hsPum2_hsAdar2_T375G_E488Q	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2 (E488Q, T375G)
250539	pCS2_hsPum2_hsAdar2	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2
250540	pCS2_HA_dreAdar1	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zebrafish Adar1
250541	pCS2_drePum1_HA_dreAdar1	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1
250542	pCS2_hsPum2_dreAdar1	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of zebrafish Adar1
250543	pCS2_RFP_1xRecEnhancedSTOP_1xBoxB_EYFP	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with one editible stop codon
250544	pCS2_RFP_1xRecEnhancedEDITED_1xBoxB_EYFP	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without an editible stop codon
250545	pCS2_RFP_2xRecEnhancedSTOP_1xBoxB_EYFP	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with two editible stop codon
250547	pCS2_RFP_2xRecEnhSTOP_EcoRI_EYFP	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with two editible stop codons
250548	pCS2_RFP_1xRecEnhSTOP_EcoRI_EYFP	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with one editible stop codon
250550	pCS2_N22_HA_hsAdar2_T375G_E488Q	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain of human Adar2 (E488Q, T375G)
250552	pCS2_EYFP_EcoRI_SV40_Recorder	In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of an empty recorder containing an EcoRI site.