RBPscan: A quantitative in vivo tool for profiling RNA-binding protein interactions.
Kretov DA, Sanborn O, McIsaac T, Park E, Imrat I, Wu S, Regis M, Harvey LM, Cifuentes D
Mol Cell. 2026 Feb 6:S1097-2765(26)00023-7. doi: 10.1016/j.molcel.2026.01.003.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250532 | pCS2_N22_HA_dreAdar1 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain on zebrafish Adar1 |
| 250533 | pCS2_N22_HA_dreAdar1_E488Q | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zerbafish Adar1 (E488Q) |
| 250534 | pCS2_EYFP_3xBoxB_Recorder_SV40 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a recorder containing 3 box-b hairpins |
| 250535 | pCS2_HA_hsAdar2 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of human Adar2 |
| 250537 | pCS2_hsAdar2_E488Q_T375G | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of human Adar2 (E488Q, T375G) |
| 250538 | pCS2_hsPum2_hsAdar2_T375G_E488Q | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2 (E488Q, T375G) |
| 250539 | pCS2_hsPum2_hsAdar2 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2 |
| 250540 | pCS2_HA_dreAdar1 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zebrafish Adar1 |
| 250541 | pCS2_drePum1_HA_dreAdar1 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1 |
| 250542 | pCS2_hsPum2_dreAdar1 | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of zebrafish Adar1 |
| 250543 | pCS2_RFP_1xRecEnhancedSTOP_1xBoxB_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with one editible stop codon |
| 250544 | pCS2_RFP_1xRecEnhancedEDITED_1xBoxB_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without an editible stop codon |
| 250545 | pCS2_RFP_2xRecEnhancedSTOP_1xBoxB_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with two editible stop codon |
| 250546 | pCS2_RFP_2xRecEnhancedEdited_1xBoxB_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without two editible stop codons |
| 250547 | pCS2_RFP_2xRecEnhSTOP_EcoRI_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with two editible stop codons |
| 250548 | pCS2_RFP_1xRecEnhSTOP_EcoRI_EYFP | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with one editible stop codon |
| 250549 | pCS2_EYFP_3xBoxB_A57N40_Recorder | In vitro transcription (SP6 promotor) of a recorder containing 3 box-b hairpins followed by A57N40 |
| 250550 | pCS2_N22_HA_hsAdar2_T375G_E488Q | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain of human Adar2 (E488Q, T375G) |
| 250551 | pCS2_EYFP_EcoRI_A57N40_Recorder | In vitro transcription (SP6 promotor) of an empty recorder containing an EcoRI site followed by A57N40 |
| 250552 | pCS2_EYFP_EcoRI_SV40_Recorder | In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of an empty recorder containing an EcoRI site. |