Modular engineering of thermoresponsive allosteric proteins.
Kroell AS, Hoffmann KH, Motzkus NA, Lemmen N, Happ N, Wolf B, von Bachmann AL, Southern N, Vogd F, Aschenbrenner S, Niopek D, Mathony J
Nat Chem Biol. 2026 Feb 12. doi: 10.1038/s41589-026-02151-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250776 | AraC-S170-LOV-C450A-D432V(M392) | Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli |
| 250777 | AraC-S170-LOV-C450A-K488N(M393) | Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli |
| 250778 | AcrIIA5-E76-LOV-C450A-K488N(M478) | Expresses AcrIIA5-E76-AsLOV2 with C450A and K488N mutation from a CMV promoter. For use in mammalian cells |
| 250779 | SpyCas9-E566-LOV-C450A-D432V(M519) | Potent temperature-deactivated SpyCas9 |
| 250780 | SpyCas9-T1048-cpGR2(#4670) | Potent chemically-activated and temperature-deactivated SpyCas9 |