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Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction.

Shen Z, Gelashvili Z, Niethammer P
Nat Cell Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
250904pSBbi-cpla2-mKate2-P2A-EGFP-KDEL Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in mammallian cell lines
250905pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
250908pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines
250912PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61BDual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
250914pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines
250915hsp70I: cPla-mKate2-P2A-EGFP-KDELDual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Antibodies from Article