Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction.
Shen Z, Gelashvili Z, Niethammer P
Nat Cell Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 250904 | pSBbi-cpla2-mKate2-P2A-EGFP-KDEL | Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in mammallian cell lines |
| 250905 | pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1 | Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines |
| 250908 | pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-LMNB1 | Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines |
| 250912 | PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B | Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines |
| 250914 | pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1 | Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines |
| 250915 | hsp70I: cPla-mKate2-P2A-EGFP-KDEL | Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock |