Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cells.
Bot JF, Zhao Z, Li M, Kammeron D, Shang P, Geijsen N
Commun Biol. 2026 Jan 19;9(1):233. doi: 10.1038/s42003-026-09511-3.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251210 | pEF-1a_dEGFP-Y66S-IRES-PuroR-WPRE | Lentiviral transfer plasmid for the generation of EF1a driven dEGFP-Y66S expressing cells. Puromycin selection marker. |
| 251211 | pEF-1a_EGFP-IRES-PuroR-WPRE | Lentiviral transfer plasmid for the generation of EF1a driven EGFP expressing cells. Puromycin selection marker. |
| 251213 | pET15b_LbuCas13a-6xHIS | Bacterial overexpression and recombinant protein purification of LbuCas13a. |
| 251214 | pET15b_LwaCas13a-6xHIS | Bacterial overexpression and recombinant protein purification of LwaCas13a. |
| 251215 | pET15b_LshCas13a-6xHIS | Bacterial overexpression and recombinant protein purification of LshCas13a. |
| 251216 | pET15b_PspCas13b-6xHIS | Bacterial overexpression and recombinant protein purification of PspCas13b. |
| 251217 | pET15b_BzCas13b-6xHIS | Bacterial overexpression and recombinant protein purification of BzCas13b. |
| 251218 | pET15b_RspCas13d-6xHIS | Bacterial overexpression and recombinant protein purification of RspCas13d. |
| 251219 | pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD | Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells. |
| 251220 | pEF-1a_RfxCas13d-HA-IRES-BFP-2A-BSD | Lentiviral transfer plasmid for the generation of EF1a driven RfxCas13d expressing cells |
| 251221 | pEF-1a_EGFP_IRES_BSD_WPRE | Lentiviral transfer plasmid for the generation of EF1a driven EGFP expressing cells |
| 251222 | pEF-1a_mScarlet_IRES_BSD_WPRE | Lentiviral transfer plasmid for the generation of EF1a driven mScarlet expressing cells |