FidlTrack: high-fidelity structure-aware single particle tracking resolves intracellular molecular motion in organelles sensing APP processing.
Parutto P, Yuan Y, Davi V, Pons-Lanau R, Ebeling S, Gupta K, Bottanelli F, Garcia-Parajo MF, Campelo F, Kaminski CF, Chambers JE, Nixon-Abell J, Avezov E
Nat Commun. 2026 Feb 11;17(1):2639. doi: 10.1038/s41467-026-69067-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251341 | pLV_CMV_mEmerald-KDEL | ER lumen targeted mEmerald, Lentiviral backbone, CMV promoter. |
| 251342 | pLV_CMV_Prss-AtagIB-Halo-KDEL | ER targeted anti-Alfatag intrabody tagged with Halotag, Lentiviral backbone, CMV promoter. |
| 251345 | CMV_4xmts_HaloTag | Mitochondria matrix targeted HaloTag with 4 mitochondria targeting sequences. CMV promoter. |
| 251346 | pLV_Tre3g_SBP_Halo_APP_SNAPf_CMV_SS_Strp_staygold_KDEL | Simultaneous expression of APP fused at N-term with Streptavidin binding protein (SBP) and HaloTag and SnapTag at C-term and ER-retained streptavidin fused to StayGold. Lentiviral backbone. |