Single-molecule fluorescence microscopy reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of live inner ear hair cells.
Miyoshi T, Vishwasrao HD, Belyantseva IA, Miyoshi J, Sajeevadathan M, Ishibashi Y, Adadey SM, Harada N, Shroff H, Friedman TB
Nat Commun. 2025 Sep 1;16(1):8149. doi: 10.1038/s41467-025-63102-0.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251419 | pHalo2X-C1 | Mammalian expression vector for expressing a protein of interest with an N-terminal HaloTag |
| 251421 | pHalo2X-actin | Mammalian expression vector for expressing human beta-actin with an N-terminal HaloTag |
| 251423 | pHalo2X-mMYO7A-HMM-FKBP | Mammalian expression vector for expressing a mouse MYO7A motor fragment with a C-terminal FKBP |
| 251424 | pHalo2X-mMYO7A-HMM-FRB | Mammalian expression vector for expressing a mouse MYO7A motor fragment with a C-terminal FRB |
| 251425 | pHalo2X-mMYO7A-Full | Mammalian expression vector for expressing HaloTag-fused full-length mouse MYO7A |
| 251426 | pHalo2X-mMYO7A-RK/AA | Mammalian expression vector for expressing a HaloTag-fused mouse MYO7A mutant that destabilizes the autoinhibitory conformation. |
| 251427 | pHalo2X-mMYO7A-ΔSH3-ΔM/F2 | Mammalian expression vector for expressing HaloTag-fused mouse MYO7A truncated after the first MyTH4-FERM domain |
| 251428 | pHalo2X-bMYO10-MD-FKBP | Mammalian expression vector for expressing a HaloTag-fused bovine monomeric MYO10 motor fragment with a C-terminal FKBP |
| 251429 | pHalo2X-bMYO10-MD-FRB | Mammalian expression vector for expressing a HaloTag-fusedbovine monomeric MYO10 motor fragment with a C-terminal FRB |
| 251430 | IL2Ralpha-pEGFP-FKBP | Mammalian expression vector for expressing the human interleukin-2 receptor alpha subunit (IL2Ralpha membrane anchor) fused to EGFP and FKBP at the C-terminus |
| 251431 | FRB-mUSH1C-DFCR-pEGFP | Mammalian expression vector for expressing the actin-binding motif of mouse harmonin b with an N-terminal FRB and a C-terminal EGFP |
| 251432 | pHA-C1 | Mammalian expression vector to express a protein of interest with an N-terminal HA tag |
| 251433 | pHA-N3 | Mammalian expression vector to express a protein of interest with a C-terminal HA tag |
| 251434 | pHA-mMYO7A-Full | Mammalian expression vector for expressing full-length mouse MYO7A with an N-terminal HA tag |
| 251435 | mMYO7A-Full-pHA | Mammalian expression vector for expressing full-length mouse MYO7A with a C-terminal HA tag |