Programmable genome editing in human cells using RNA-guided bridge recombinases

Pelea O, Tálas A, Carrera JF, Mathis N, van de Venn L, Yeh CD, Kulcsár PI, Marquart KF, Weber Y, Gerecke SE, Harvey-Seutcheu IF, Mailänder D, Pfleiderer MM, Chanez C, Corn JE, Schwank G, Jinek M
Science, 2026. eadz1884, doi:10.1126/science.adz1884 Article

Plasmids from Article

ID	Plasmid	Purpose
251493	CMV_3xFLAG_NLS_ISCro4_NLS_SV40_polyA	Expression of a human codon optimised ISCro4 bridge recombinase
251494	CMV_3xFLAG_NLS_ISCro4_NLS_T2A_puroR_SV40_polyA	Expression of a human codon optimised ISCro4 bridge recombinase
251495	U6_ISCro4_(WT)_full-length_bridge_RNA_polIII_term	Expression of the native full length ISCro4 bridge RNA
251496	U6_ISCro4_(WT)_TBL_bridge_RNA_polIII_term	Expression of the Target Binding Loop (TBL) component of the native ISCro4 bridge RNA
251497	U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term	Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA
251498	Tol2_ISCro4_Deletion_Reporter	Reporter plasmid for monitoring deletion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components
251499	Tol2_ISCro4_Inversion_Reporter	Reporter plasmid for monitoring inversion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components
251500	Tol2_ISCro4_Insertion_Reporter	Reporter plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and pDonor_ISCro4_(WT)_Donor_ECFP plasmid
251501	pDonor_ISCro4_(WT)_Donor_ECFP	Donor plasmid for monitoring insertion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL and Tol2_ISCro4_Insertion_Reporter plasmid
251502	T7_MBP_TEV_ISCro4_6xHis_T7_term	Bacterial expression vector for producing recombinant ISCro4 (E. coli codon optimised)