Engineered base editors with reduced bystander editing through directed evolution.
Perrotta RM, Vinke S, Ferreira R, Moret M, Mahas A, Chiappino-Pepe A, Riedmayr LM, Mehra AT, Lehmann LS, Church GM
Nat Biotechnol. 2025 Dec 18. doi: 10.1038/s41587-025-02937-w.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251544 | ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY) | Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain |
| 251545 | ABEx2_SpRY (ABE8e_L34W_nCas9_SpRY) | Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain |
| 251546 | ABEx3_SpRY (ABE8e_M151E_nCas9_SpRY) | Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain |
| 251547 | ABEx4_SpRY (ABE8e_V28C_M151E_nCas9_SpRY) | Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain |
| 251548 | ABEx1_WT_GFP (ABE8e_V28C_nCas9_WT) | Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain |
| 251549 | ABEx2_WT_GFP (ABE8e_L34W_nCas9_WT) | Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the L34W mutation in the deaminase domain |
| 251550 | ABEx3_WT_GFP (ABE8e_M151E_nCas9_WT) | Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the M151E mutation in the deaminase domain |
| 251551 | ABEx4_WT_GFP (ABE8e_V28C_M151E_nCas9_WT) | Mammalian expression plasmid encoding an nCas9-WT adenine base editor (ABE8e) carrying the V28C and M151E mutations in the deaminase domain |
| 251552 | ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W) | Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain |