Rapid fluorescence lifetime sensor development of LifeCamp enables transient and baseline absolute calcium measurements
Lodder B, Raghubardayal M, Ganesh S, Cai X, Stern J, Sherman M, Rosen P, Kamath T, Hartman I, Siegel M, Timmins J, Adan R, Andermann M, Sabatini BL
bioRxiv 2025.12.23.696288
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251723 | CAG-LifeCamp | Expression of calcium lifetime sensor LifeCamp under CAG promoter |
| 251724 | pAAV-hsyn-LifeCamp | Expression of calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone |
| 251725 | pAAV-hsyn-LifeCamp0 | Expression of binding mutant control calcium lifetime sensor LifeCamp0 under hSyn promoter in pAAV backbone |
| 251726 | pAAV-hsyn-DIO-LifeCamp | Expression of Cre-dependent calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone |
| 251727 | pAAV-hsyn-DIO-LifeCamp0 | Expression of Cre-dependent binding mutant control calcium lifetime sensor LifeCamp0 under hSyn promoter in pAAV backbone |
| 251728 | pAAV-hsyn-RiboL1-LifeCamp | Expression of soma-targeted calcium lifetime sensor LifeCamp under hSyn promoter in pAAV backbone |