Identification and engineering of highly functional potyviral proteases in cells using co-evolutionary models
Lim Suan MB, Ziegler C, Syed Z, Yedavalli AS, Nagineni J, Raposo R, Tunikipati A, Kaur J, Morcos F, Dingal DP
Nat Commun 17, 3257 (2026).
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 251767 | pDD2270 pCS2-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10) | TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay |
| 251768 | pDD1707 pCS2-NES-arrb2a-(DYDIPTTENLYFQ/SGTVDAG)x1-SV40NLS-sfCherry3C(1-10) | TEVp substrate (extended motif) for fluorescent protein reconstitution assay |
| 251769 | pDD3254 pCS2-NES-arrb2a-GGSGGSG-ENLYFQ/S-GGSGGS-SV40NLS-sfCherry3C(1-10) | TEVp substrate (GS-flanked motif) for fluorescent protein reconstitution assay |
| 251770 | pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10) | Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay |
| 251771 | pDD3233 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls-p2a-TEVp | Nuclear reporter for fluorescent protein reconstitution assay-p2a-TEVp |
| 251772 | pDD0257 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls | Nuclear reporter for fluorescent protein reconstitution assay |
| 251773 | pDD0316 pCS2-TEVp | Potyviral protease specific against heptapeptide sequence: ENLYFQS |
| 251774 | pDD0317 pCS2-TVMVp | Potyviral protease specific against heptapeptide sequence: ETVRFQS |
| 251775 | pDD0318 pCS2-PSbMVp | Potyviral protease specific against heptapeptide sequence: IKVRLQA |
| 251776 | pDD0320 pCS2-NDVp | Potyviral protease specific against heptapeptide sequence: QRVSFQS |
| 251777 | pDD0321 pCS2-WTMVp | Potyviral protease specific against heptapeptide sequence: LDVRHQS |
| 251778 | pDD0322 pCS2-SuMMVp | Potyviral protease specific against heptapeptide sequence: EEIHLQG |
| 251779 | pDD0323 pCS2-SYSVp | Potyviral protease specific against heptapeptide sequence: SYVSYQA |
| 251780 | pDD0324 pCS2-PPVp | Potyviral protease specific against heptapeptide sequence: NVVVHQA |
| 251781 | pDD0326 pCS2-PeMoVp | Potyviral protease specific against heptapeptide sequence: DEVRYQS |
| 251782 | pDD0327 pCS2-OYDVp | Potyviral protease specific against heptapeptide sequence: KEVRYQA |
| 251783 | pDD0328 pCS2-ClYVVp | Potyviral protease specific against heptapeptide sequence: MKFVFQS |
| 251784 | pDD0904 pCS2-KeuMVp | Potyviral protease specific against heptapeptide sequence: DVVTLQA |
| 251785 | pDD0905 pCS2-AWMVp | Potyviral protease specific against heptapeptide sequence: LVVYHQA |
| 251786 | pDD0907 pCS2-PTVp | Potyviral protease specific against heptapeptide sequence: DEVYHQA |
| 251787 | pDD0910 pCS2-ZYMVp | Potyviral protease specific against heptapeptide sequence: DTVMLQS |
| 251788 | pDD0915 pCS2-KoMVp | Potyviral protease specific against heptapeptide sequence: EQVYHQS |
| 251789 | pDD0916 pCS2-LMVp | Potyviral protease specific against heptapeptide sequence: DEVYHQV |
| 251790 | pDD0918 pCS2-EAPVp | Potyviral protease specific against heptapeptide sequence: ESVSLQT |
| 251791 | pDD0920 pCS2-SPV2p | Potyviral protease specific against heptapeptide sequence: EPVYHQS |
| 251792 | pDD0921 pCS2-SPFMVp | Potyviral protease specific against heptapeptide sequence: LEVYHQS |
| 251793 | pDD0922 pCS2-TVBMVp | Potyviral protease specific against heptapeptide sequence: EEVVHQN |
| 251794 | pDD0923 pCS2-CABMVp | Potyviral protease specific against heptapeptide sequence: EDVVLQS |
| 251795 | pDD0929 pCS2-LMoVp | Potyviral protease specific against heptapeptide sequence: EIVAFQA |
| 251796 | pDD0930 pCS2-ScMVp | Potyviral protease specific against heptapeptide sequence: VEVYHQA |
| 251797 | pDD1773 pCS2-PMMVp | Potyviral protease specific against heptapeptide sequence: EPMKFQV |
| 251798 | pDD1775 pCS2-ISMVp | Potyviral protease specific against heptapeptide sequence: INVRFEG |
| 251799 | pDD1776 pCS2-PSp | Potyviral protease specific against heptapeptide sequence: EIVDFQA |
| 251800 | pDD1777 pCS2-PMAVp | Potyviral protease specific against heptapeptide sequence: DSITFQA |
| 251802 | pDD1779 pCS2-KeuMV2p | Potyviral protease specific against heptapeptide sequence: DVVTLQS |
| 251803 | pDD1780 pCS2-ACPp | Potyviral protease specific against heptapeptide sequence: ESVSLQS |