Gene syntax defines supercoiling-mediated transcriptional feedback.
Johnstone CP, Love KS, Kabaria SR, Jones R, Blanch-Asensio A, Ploessl DS, Peterman EL, Lee R, Yun J, Oakes CG, Mummery CL, Davis RP, DeKosky BJ, Zandstra PW, Galloway KE
bioRxiv [Preprint]. 2025 Jan 19:2025.01.19.633652. doi: 10.1101/2025.01.19.633652.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252538 | aRJ_00134_PGK-mNeonGreen-syn_pA-EF1alpha-TagBFP-syn_pA | Constitutive promoters with downstream tandem syntax for PiggyBac integration |
| 252539 | aRJ_00135_EF1alpha-TagBFP-syn_pA-PGK-mNeonGreen-syn_pA | Constitutive promoters with upstream tandem syntax for PiggyBac integration |
| 252540 | pKG01701_CMV-rtTA-betaglobin_pA-CMV-PuroR-bGH | Secondary PiggyBac vector for puromycin selection and expression of transcriptional activator |
| 252541 | pKG01416_TRE-TagBFP-bGH-EF1alpha-mRuby2-SV40 | Inducible gene and constitutive reporter with downstream tandem syntax, used to generate monoclonal PiggyBac-integrated lines |
| 252542 | pKG01417_TRE-TagBFP-bGH-(EF1alpha-mRuby2-SV40) | Inducible gene and constitutive reporter with convergent syntax, used to generate monoclonal PiggyBac-integrated lines |
| 252543 | pKG01418_(TRE-TagBFP-bGH)-EF1alpha-mRuby2-SV40 | Inducible gene and constitutive reporter with divergent syntax, used to generate monoclonal PiggyBac-integrated lines |
| 252544 | pKG04154_Bxb1-GT_(TRE3G-mScarlet-WPRE)-CAG-rtTA-T2A-mTagBFP2-bGH | Inducible Tet all-in-one circuit with divergent syntax, STRAIGHT-IN donor targeting Bxb1 GT landing pad |
| 252545 | pKG04157_Bxb1-GT_CAG-rtTA-T2A-mTagBFP2-bGH-TRE3G-mScarlet-WPRE | Inducible Tet all-in-one circuit with upstream tandem syntax, STRAIGHT-IN donor targeting Bxb1 GT landing pad |
| 252546 | pKG04155_Bxb1-GT_CAG-rtTA-T2A-mTagBFP2-bGH-(TRE3G-mScarlet-WPRE) | Inducible Tet all-in-one circuit with convergent syntax, STRAIGHT-IN donor targeting Bxb1 GT landing pad |
| 252547 | pKG04062_pLentiX1-EF1alpha-GapR-3xFLAG-NLS-EGFP-bGH | Lentiviral expression of GapR-EGFP fusion protein for GapRUN assay |
| 252548 | pKG02752_pLentiX1-EFS-rtTA-P2A-mGL-WPRE | Lentiviral expression of the rtTA transcriptional activator with a fluorescent reporter |
| 252549 | pKG01522_pLentiX1-CMV-rtTA-betaglobin_pA | Lentiviral expression of the rtTA transcriptional activator |
| 252550 | pKG03426_pLentiX1-EF1alpha-mRuby2-bGH-TRE-TagBFP-bGH | Inducible gene and constitutive reporter with upstream tandem syntax, lentiviral vector |
| 252551 | pKG03425_pLentiX1-TRE-TagBFP-bGH-EF1alpha-mRuby2-bGH | Inducible gene and constitutive reporter with downstream tandem syntax, lentiviral vector |
| 252552 | pKG03427_pLentiX1-EF1alpha-mRuby2-bGH-(TRE-TagBFP-bGH) | Inducible gene and constitutive reporter with convergent syntax, lentiviral vector |
| 252553 | pKG03428_pLentiX1-(TRE-TagBFP-bGH)-EF1alpha-mRuby2-bGH | Inducible gene and constitutive reporter with divergent syntax, lentiviral vector |
| 252554 | pKG01484_pLentiX1-TRE-mRuby2-bGH-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with downstream tandem syntax, lentiviral vector |
| 252555 | pKG01487_pLentiX1-(TRE-mRuby2-bGH)-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with divergent syntax, lentiviral vector |
| 252556 | pKG03549_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-TRE-mRuby2-bGH | Inducible Tet all-in-one circuit with upstream tandem syntax, lentiviral vector |
| 252557 | pKG03572_pLentiX1-(TRE-mRuby2-bGH)-cHS4.core-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 core insulator, lentiviral vector |
| 252558 | pKG03571_pLentiX1-TRE-mRuby2-bGH-cHS4.core-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with downstream tandem syntax and intergenic cHS4 core insulator, lentiviral vector |
| 252559 | pKG03574_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-cHS4.core-TRE-mRuby2-bGH | Inducible Tet all-in-one circuit with upstream tandem syntax and intergenic cHS4 core insulator, lentiviral vector |
| 252560 | pKG03576_pLentiX1-(TRE-mRuby2-bGH)-cHS4.full-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with divergent syntax and intergenic cHS4 full insulator, lentiviral vector |
| 252561 | pKG03575_pLentiX1-TRE-mRuby2-bGH-cHS4.full-EFS-rtTA-P2A-mGL-WPRE | Inducible Tet all-in-one circuit with downstream tandem syntax and intergenic cHS4 full insulator, lentiviral vector |
| 252562 | pKG03578_pLentiX1-EFS-rtTA-P2A-mGL-WPRE-cHS4.full-TRE-mRuby2-bGH | Inducible Tet all-in-one circuit with upstream tandem syntax and intergenic cHS4 full insulator, lentiviral vector |