Efficient Transgene-Free Multiplexed Germline Editing via Viral Delivery of an Engineered TnpB.
Weiss T, Kamalu M, Shi H, Wirnowski G, Ingelsson A, Amerasekera J, Vohra K, Trinidad MI, Li Z, Freitas E, Steinmetz N, Ambrose C, Chen K, Doudna JA, Jacobsen SE
Plant Biotechnol J. 2026 Mar 17. doi: 10.1111/pbi.70644.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252576 | pTW2658 | TRV2 multiplex vector expressing Ymu1-WFR, AtCHLl1 gRNA4 and AtPDS3 gRNA12 |
| 252577 | pTW2657 | TRV2 multiplex vector expressing Ymu1-WFR, AtPDS3 gRNA12 and AtCHLl1 gRNA4 |
| 252579 | pTW2278 | TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA4 and AtPDS3 gRNA12 |
| 252580 | pTW2279 | TRV2 multiplex vector expressing wild type Ymu1, AtPDS3 gRNA12 and AtCHLl1 gRNA4 |
| 252581 | pTW2498 | TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA6 and AtCHLl1 gRNA4 |
| 252582 | pTW2652 | TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA4 |
| 252583 | pTW2653 | TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA6 |
| 252584 | pTW2660 | TRV2 expressing Ymu1-WFR protein and AtCHLl1 gRNA9 |
| 252585 | pTW2684 | ccdb dropout cloning vector for PaqCI Golden Gate assembly of Ymu1-WFR guides into TRV2 |