Multi-kinesin clusters impart mechanical stress that reveals mechanisms of microtubule breakage in cells.
Geng Q, Bonilla A, Sandwith SN, Verhey KJ
J Cell Biol. 2025 Oct 6;224(10):e202501070. doi: 10.1083/jcb.202501070. Epub 2025 Aug 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253321 | pN1_FKBP-HsKIF1C(349-1103)-mCh | mammalian expression of the stalk+tail domains (aa 349-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins. |
| 253322 | pN1_FKBP-HsKIF1C(886-1103)-mCh | mammalian expression of the IDR domain (aa 886-1103) of HsKIF1C tagged with FKBP and mCherry. The FKBP tag enables rapamycin-induced attachment to FRB-tagged proteins. |
| 253323 | pN1_FKBP-mCh-FTH1 | mammalian expression of human FTH1 tagged with FKBP and mCherry. FTH1 self-assembles into a 24-merparticle. FKBP enables rapamycin-induced attachment to FRB-tagged proteins. |
| 253324 | pN1_HsKIF1C-mNG | mammalian expression of full-length human KIF1C (aa 1-1103) tagged with mNG. |
| 253325 | pN1_HsKIF1C(1-376)-NC-LZ-mNG | mammalian expression of the motor domain (aa 1-376) of human KIF1C tagged with mNG. The NC and LZ sequences facilitate stable dimerization of the motor domains. |
| 253326 | pN1_HsKIF1C(1-376)-NC-LZ-TagBFP-FRB | mammalian expression of the motor domain of human KIF1C tagged with TagBFP and FRB. NC and LZ facilitate stable dimerization. FRB enables rapamycin-induced attachment to FKBP-tagged proteins. |
| 253327 | pN1_HsKIF1C(349-1103)-mNG | mammalian expression of the stalk+tail domains of human KIF1C (aa 349-1103) tagged with mNG. |
| 253328 | pN1_HsKIF1C(886-1103)-mNG | mammalian expression of the IDR domain of human KIF1C (aa 886-1103) tagged with mNG. |