A split ALFA tag-nanobody system for protein localization and proximity proteomics in mycobacteria.

Fay A, Kurland AP, Li Z, Monetti M, Johnson JR, Glickman MS
mBio. 2025 Aug 13;16(8):e0097125. doi: 10.1128/mbio.00971-25. Epub 2025 Jun 27. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
252906	pAJF1131	T7-pelB-NBALFA-LE-6his amp (for purification of his-tagged NBALFA from E. coli)
252907	pAJF1137	Tet-inducible TurboID-NBALFA fusion plasmid. Contains oriM (mycobacterial origin of replication) and kanamycin-resistance cassette.