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DOT1L provides transcriptional memory through PRC1.1 antagonism.

Neville D, Ferguson DT, Heikamp EB, Lai Z, Magor GW, Lam C, Dobbs OG, Levina V, Knezevic K, The JJ, Alex S, Suits SC, Rumler B, Uckelmann M, Talarmain L, Lam EYN, Perkins AC, Armstrong SA, Bell CC, Davidovich C, Gilan O
Nat Cell Biol. 2026 Feb 3. doi: 10.1038/s41556-025-01859-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
252955pET3_H3.1_C96S_C110A_K79CExpression of human histone 3.1 mutant for generating lysine analogue H3.1_K79me2/3
252956pMB.PRC1.1_RING1B_MBP-PCGF1Expression of PRC1.1 core (RING1B and PCGF1) in insect cells.
252957pMB.PRC1.4_RING1B_MBP-PCGF4Expression of PRC1.4 core (RING1B and PCGF4/BMI1) in insect cells.

Antibodies from Article