The dynamic response of the bacterial flagellar motor to its direct intracellular input signal.
Vrabioiu AM, Hosu BG, Samuel ADT
Proc Natl Acad Sci U S A. 2026 Mar 10;123(10):e2516278123. doi: 10.1073/pnas.2516278123. Epub 2026 Mar 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253255 | pA-Opto | Opto-CheY expressed using the proA promoter and T7 RBS variant GAAGGAGgT |
| 253256 | pA-Opto_N449S | Opto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgT |
| 253257 | pA-Opto_V416T | Opto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgT |
| 253258 | pA_higher-Opto_N449S | Opto-CheY N449S expressed using the proA promoter and wild type T7 RBS |
| 253259 | pA_higher-CheY-cpAsLOV2_N449S | Encodes the fusion of CheY and cpAsLOV2 N449S driven with the proA promoter and wild type T7 RBS |
| 253260 | pA_higher-CheY-pMag-mNeonGreen | Encodes the fusion of CheY, pMag, and mNeonGreen driven with the proA promoter and wild type T7 RBS |
| 253285 | p5g-Opto_N449S | Opto-CheY N449S expressed using the pro5 promoter and T7 RBS variant GAAGGAGgT |
| 253286 | pT-Sticky | pTrc99A with FlgE_RRR ( 'sticky' hook) insert |