Accessing intractable, phosphorylated intrinsically disordered proteins via a protease-cleavable inclusion body tag.
Vesely CH, Stanisheuski S, Lien E, Reardon P, Chung PJ, Mehl RA, Cooley RB
Protein Sci. 2026 Apr;35(4):e70520. doi: 10.1002/pro.70520.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253344 | pRBC-PINBodyC-Tau(297-441)-WT-his6 | Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441 |
| 253345 | pRBC-PINBodyC-Tau(297-441)-404 TAG-his6 | Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 297-441, TAG codon at residue 404 for phosphoserine and non-hydrolyzable analog of phosphoserine encoding |
| 253346 | pRBC-PINBodyN-Tau(1-441)-WT | Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 1-441 |
| 253347 | pRBC-PINBodyN-Tau(1-441)-404TAG | Express in E.coli, Inclusion body tagged protein expression, Tau 2N4R residues 1-441, TAG codon at residue 404 for phosphoserine encoding |