Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow.
Seong MJ, Kim H, Lee H, Kim H, Lee SG, Lee MJ, Jun JS, Hong S, Kim TH, Kim SK, Shin J, Lee DH
Trends Biotechnol. 2026 Jan 14:S0167-7799(25)00502-5. doi: 10.1016/j.tibtech.2025.12.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254020 | Modified pYTK075 | A part plasmid designed to use the yeast selective marker (LEU2 gene) as a Level 1 TU fragment. |
| 254021 | Modified pYTK048 | It was constructed by reversing the orientation of the BsaI sites in the original pYTK048 and is used for the construction of connectors. |
| 254023 | pConnector_L1-R2 | A plasmid containing the L1-R2 connector. |
| 254024 | pConnector_L2-R3 | A plasmid containing the L2-R3 connector. |
| 254025 | pConnector_L2-RE | A plasmid containing the L2-RE connector. |
| 254026 | pConnector_L3-R4 | A plasmid containing the L3-R4 connector. |
| 254027 | pConnector_L4-R5 | A plasmid containing the L4-R5 connector. |
| 254029 | pConnector_L5-R6 | A plasmid containing the L5-R6 connector. |
| 254031 | pConnector_L7-R8 | A plasmid containing the L7-R8 connector. |
| 254033 | pConnector_L8-RE | A plasmid containing the L8-RE connector. |