A novel RAB5 binding site in human VPS34-CII that is likely the primordial site in eukaryotic evolution.
Špokaitė S, Ohashi Y, Bourguet M, Dessus AN, Williams RL
eLife
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235711 | pYO1344 | Expression of FL human VPS34 R199E E200R E202R mutant in mammalian cells |
| 235713 | pYO1356 | Expression of FL human VPS34 R199E E200R E202R muntant-EGFP in mammalian cells |
| 235714 | pYO1846 | Expression of mCherry-human Beclin 1 in mammalian cells |
| 235715 | pYO1847 | Expression of FL human VPS15 S579D_H580D_T583E mutant -ZZ in mammalian cells |
| 235716 | pYO1865 | Expression of FL human VPS15 S579D_H580D_T583E mutant in mammalian cells |
| 235720 | pYO634 | Expression of FL yeast VPS15-EGFP under its own promoter in yeast |
| 235721 | pYO1880 | Expression of FL yeast VPS15 S622D_H623D_T626E mutant -EGFP under its own promoter in yeast |
| 235722 | pYO950 | Expression of FL yeast VPS15-3x FLAG under its own promoter in yeast |
| 235723 | pYO1877 | Expression of FL yeast VPS15 S622D_H623D_T626E mutant-3x FLAG under its own promoter in yeast |
| 235739 | pYO1510 | Protein expression of mCherry-14 x His in bacteria |
| 235767 | pYO1920 | Protein expression of ScVPS34 untagged + ScVPS15 S622D_H623E_T626E-ZZ in yeast |
| 235768 | pYO1922 | Protein expression of ScVPS38+ScVPS30-mCherry |
| 235769 | pYO1919 | Protein expression of 6xHis ScVPS21 (Q66L)-EGFP |
| 242480 | pYO1879 | Integrate dRFP-ScVPS21 Q66L into the yeast genome with LEU2 marker |