Le Cong Lab: Surfaceome CRISPR activation screening uncovers ligands regulating tumor sensitivity to NK cell killing
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 255456 | pLV EF1a Fluc2 2A GFP 2A BSD | Expresses firefly luciferase, eGFP, and blasticidin deaminase from a lentiviral vector |
| 255457 | AAVS1 BSD EF1a Fluc2 2A GFP | AAVS1 knock-in construct that expresses firefly luciferase, eGFP, and blasticidin deaminase |
| 255458 | pLV EF1a dCas9VP64 2A EGFP 2A BSD | Expresses dCas9VP64, eGFP, and blasticidin |
| 255459 | pLV Ef1a 2A PuroR 2A BFP | Allows for facile cloning of an insert cDNA upstream of Puromycin resistance and BFP markers |
| 255460 | pLV EF1a 2A HygroR 2A mKate | Allows for facile cloning of an insert cDNA upstream of hygromycin resistance and mKate markers |
| 255461 | lentiCRISPRi v3 | Expresses sgRNA2.1 cassette, dCas9-Zim3, and puromycin resistance |
| 255462 | pLV EFS PCP-p65-HSF1 2A mcherry2 2A HygR | Expresses SAM components PCP-p65-HSF1, mcherry2, and hygromycin resistance |
| 255463 | pLV U6 sgRNA2.0 pp7 EFS Puro 2A.BFP | Expresses Puro and BFP and has an sgRNA2.0 scaffold for insertion of CRISPR activation gRNAs |
| 255499 | eSpCas9(1.1)-T2A-GFP - GlntRNA | Expresses eSpCas9(1.1) with GFP and a GlntRNA promoter for gRNA expression |