Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi.

Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe SW
Nat Biotechnol. 2011 Jan . 29(1):79-83. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
27990	TRMPV-Neo	Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
27991	TRMPV-ns	Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
27993	TtTMPV-Neo	Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains TurboRFP and Venus fluorescent reporters.
27994	TRMPVIR	Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
27995	TtRMPVIR	Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains DsRed2 and Venus fluorescent reporters.
27996	TRMPV-Hygro	Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.