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The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway.
Mitchell SF, Walker SE, Algire MA, Park EH, Hinnebusch AG, Lorsch JR
Mol Cell. 2010 Sep 24;39(6):950-62.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
37223pUC19-RPL41A
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37224pUC19-DAD4
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37225pTYB1-eIF4A
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37226pTYB2-eIF4B
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37227pTYB2-eIF4B-A130C
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37228pET15b-eIF4E
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37229pET15b-eIF4E-S200C
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37230pET21c-eIF4A
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37231pGEX4T1-GST-eIF4G1-His6
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37232pET-DUET-GST-eIF4G1-His6, eIF4E
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37233pTYB2-HCR1
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Antibodies from Article