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Activation of cap dependent translation by mucosal Human Papillomavirus E6 proteins is dependent on the integrity of the LXXLL binding motif.

Spangle J, Ghosh-Choudhury N, Munger K
J Virol. 2012 May 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
37433pB-actin 16E6no*
37434pB-actin 18E6
37435pB-actin 6bE6
37436pB-actin 11E6
37437pB-actin 5E6
37438pB-actin 8E6
37444pLentiNControl lentiviral plasmid containing FLAG-HA tags
37445pLentiN 16E6no*Lentiviral expression of HPV 16E6no* in mammalian cells
37446pLentiN 18E6no*Lentiviral expression of HPV 18E6no* in mammalian cells
37447pLentiN 6bE6Lentiviral expression of HPV 6bE6 in mammalian cells
37448pLentiN 11E6Lentiviral expression of HPV 11E6 in mammalian cells
37449pLentiN 5E6Lentiviral expression of HPV 5E6 in mammalian cells
37450pLentiN 8E6Lentiviral expression of HPV 8E6 in mammalian cells
37454pNCMV 16E6no*Expression of HPV 16E6no* in mammalian cells
37455pNCMV 11E6Expression of HPV 11E6 in mammalian cells
37456pNCMV 18E6no*Expression of HPV 18E6no* in mammalian cells
37457pNCMV 6bE6Expression of HPV 6bE6 in mammalian cells
37459pNCMV 5E6Expression of HPV 5E6 in mammalian cells
37460pNCMV 8E6Expression of HPV 8E6 in mammalian cells
47678pNCMV 16E6no* delPDZPDZ-binding motif is deleted
47705pNCMV 16E6no* Y54DDefective for p53 binding
47706pNCMV 16E6no* I128TDefective for E6AP binding
47800pNCMV 6bE6 L111QDefective for E6AP binding
47861pNCMV 6bE6 I127TDefective for E6AP binding
47862pNCMV 6bE6 L129TDefective for E6AP binding
47863pNCMV 11E6 L111QDefective for E6AP binding
47864pNCMV 11E6 I127TDefective for E6AP binding
47866pNCMV 18E6no* I130TDefective for E6AP binding
47887pNCMV 18E6no* delPDZPDZ-binding motif is deleted

Antibodies from Article