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Novel fluorescent genome editing reporters for monitoring DNA repair pathway utilization at endonuclease-induced breaks.
Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
Nucleic Acids Res. 2013 Oct 10.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
45564pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFPExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequence
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45565pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFPExpresses I-Sce I nuclease in mammalian cells and BFP as a tracker
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45574pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFPExpresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone
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45575pCVL.Active/Repressed TLR (Sce)Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease
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45576pCVL.SSA TLR (Sce)Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone
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45577pCVL.SSA TLR (Ani)Codes for the SSA-TLR with the target site for I-Ani I in a lentiviral backbone
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45578pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFPExpresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker
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45579pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFPExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker
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46941pCVL.SSA TLR (CCR5 TALEN Sce Spacer)Codes for the SSA-TLR with the target site for the CCR5 TALEN with the spacer region corresponding to I-Sce I in a lentiviral backbone
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Recombinant Antibodies from Article