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pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
(Plasmid #45579)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45579 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCVL
  • Backbone size w/o insert (bp) 5071
  • Total vector size (bp) 7671
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    coY2 Ani K227M
  • Alt name
    Y2 Ani Nickase
  • Insert Size (bp)
    762
  • Mutation
    co indicates codon optimization for mammalian expression. K227M mutation confers nickase activity
  • Promoter SFFV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • NLS (N terminal on backbone)
    • IRES BFP (C terminal on insert)

Cloning Information

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP was a gift from Andrew Scharenberg (Addgene plasmid # 45579 ; http://n2t.net/addgene:45579 ; RRID:Addgene_45579)
  • For your References section:

    Novel fluorescent genome editing reporters for monitoring DNA repair pathway utilization at endonuclease-induced breaks. Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT. Nucleic Acids Res. 2013 Oct 10. 10.1093/nar/gkt872 PubMed 24121685