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Recombinant reconstitution of sumoylation reactions in vitro.
Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F
Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53139pET23a-His-hRanGAP1tailE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag
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53140pGEX3x-hRanBP2-(IR1+M)E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity
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53141pET28a-HisYFP-Sp100E.coli expression of HisYFP tagged human Sp100 fragment
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53142pET11a-SUMO2E.coli expression of untagged mature human SUMO2 (amino acids 1-92)
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53143pET11a-SUMO3E.coli expression of untagged mature human SUMO3 (amino acids 1-93)
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Antibodies from Article