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(Plasmid #53139)


Item Catalog # Description Quantity Price (USD)
Plasmid 53139 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4196
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    Ran GTPase activating protein 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    C-terminal fragment (amino acids 398-587)
  • GenBank ID
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a-His-hRanGAP1tail was a gift from Frauke Melchior (Addgene plasmid # 53139 ; ; RRID:Addgene_53139)
  • For your References section:

    Recombinant reconstitution of sumoylation reactions in vitro. Flotho A, Werner A, Winter T, Frank AS, Ehret H, Melchior F. Methods Mol Biol. 2012;832:93-110. doi: 10.1007/978-1-61779-474-2_5. 10.1007/978-1-61779-474-2_5 PubMed 22350878