Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs.
Kirchmaier S, Lust K, Wittbrodt J
PLoS One. 2013 Oct 7;8(10):e76117. doi: 10.1371/journal.pone.0076117.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 49280 | pGGEV_+1_Linker | Golden Gate entry vector at position 1,small linker insert, plus 5' ATG |
| 49281 | pGGEV_-1_Linker | Golden Gate entry vector at position 1, small linker insert, no 5' ATG |
| 49282 | pGGEV_2_Linker | Golden Gate entry vector at position 2, small linker insert |
| 49283 | pGGEV_3_Linker | Golden Gate entry vector at position 3, small linker insert |
| 49284 | pGGEV_4_Linker | Golden Gate entry vector at position 4, small linker insert |
| 49285 | pGGEV_5_Linker | Golden Gate entry vector at position 5, small linker insert |
| 49286 | pGGEV_6_Linker | Golden Gate entry vector at position 6, small linker insert |
| 49287 | pGGEV_7_Linker | Golden Gate entry vector at position 7, small linker insert |
| 49288 | pGGEV_2'_Linker | Golden Gate entry vector at position 2, small linker insert, final entry vector for the assembly |
| 49289 | pGGEV_3'_Linker | Golden Gate entry vector at position 3, small linker insert, final entry vector for the assembly |
| 49290 | pGGEV_4'_Linker | Golden Gate entry vector at position 4, small linker insert, final entry vector for the assembly |
| 49291 | pGGEV_5'_Linker | Golden Gate entry vector at position 5, small linker insert, final entry vector for the assembly |
| 49292 | pGGEV_6'_Linker | Golden Gate entry vector at position 6, small linker insert, final entry vector for the assembly |
| 49293 | pGGEV_7'_Linker | Golden Gate entry vector at position 7, small linker insert, final entry vector for the assembly |
| 49294 | pGGEV_8'_Linker | Golden Gate entry vector at position 8, small linker insert, final entry vector for the assembly |
| 49295 | pGGEV_+1_XcmI-LacZ | Golden Gate entry vector at position 1, plus 5' ATG, LacZ |
| 49296 | pGGEV_-1_XcmI-LacZ | Golden Gate entry vector at position 1, no 5' ATG, LacZ |
| 49297 | pGGEV_2_XcmI-LacZ | Golden Gate entry vector at position 2, LacZ |
| 49298 | pGGEV_3_XcmI-LacZ | Golden Gate entry vector at position 3, LacZ |
| 49299 | pGGEV_4_XcmI-LacZ | Golden Gate entry vector at position 4, LacZ |
| 49300 | pGGEV_5_XcmI-LacZ | Golden Gate entry vector at position 5, LacZ |
| 49301 | pGGEV_6_XcmI-LacZ | Golden Gate entry vector at position 6, LacZ |
| 49302 | pGGEV_7_XcmI-LacZ | Golden Gate entry vector at position 7, LacZ |
| 49303 | pGGEV_2'_XcmI-LacZ | Golden Gate entry vector at position 2, LacZ, final entry vector for the assembly |
| 49304 | pGGEV_3'_XcmI-LacZ | Golden Gate entry vector at position 3, LacZ, final entry vector for the assembly |
| 49305 | pGGEV_4'_XcmI-LacZ | Golden Gate entry vector at position 4, LacZ, final entry vector for the assembly |
| 49306 | pGGEV_5'_XcmI-LacZ | Golden Gate entry vector at position 5, LacZ, final entry vector for the assembly |
| 49307 | pGGEV_6'_XcmI-LacZ | Golden Gate entry vector at position 6, LacZ, final entry vector for the assembly |
| 49308 | pGGEV_7'_XcmI-LacZ | Golden Gate entry vector at position 7, LacZ, final entry vector for the assembly |
| 49309 | pGGEV_8'_XcmI-LacZ | Golden Gate entry vector at position 8, LacZ, final entry vector for the assembly |
| 49310 | pGGEV_-1_FRT_+1_OA- | Golden Gate entry vector: Position 1, FRT site |
| 49311 | pGGEV_3_FRT_+1_OA- | Golden Gate entry vector: Position 3, FRT site |
| 49312 | pGGEV_2_+nls-eGFP-HA-_+1_BK+ | Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon |
| 49313 | pGGEV_4'_+nls-mCherry-Flag-_+1_BK+ | Golden Gate entry vector: Position 4', Flag tagged nls-mCherry with start and stop codon |
| 49314 | pGGW_SM_BglII | Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert |
| 49315 | pGGW_SM_BamHI | Subcloning vector for Golden Gate-based site-directed mutagenesis; BamHI and KpnI to release the insert |
| 49316 | p3E_GGWDest- | Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning |
| 49317 | p3E_GGWDest+ | Destination vector for Golden Gate assembly; 3' entry vector for Gateway cloning |
| 49318 | p5E_GGWDest- | Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning |
| 49319 | p5E_GGWDest+ | Destination vector for Golden Gate assembly; 5' entry vector for Gateway cloning |
| 49320 | pME_GGWDest- | Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning |
| 49321 | pME_GGWDest+ | Destination vector for Golden Gate assembly; middle entry vector for Gateway cloning |
| 49322 | pGGDestSC-ATG | Destination vector for Golden Gate assembly; Few restriction sites for subcloning. |
| 49323 | pGGDestSC+ATG | Destination vector for Golden Gate assembly; Few restriction sites for subcloning. |