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A genetically encoded reporter for real-time imaging of cofilin-actin rods in living neurons.

Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB, Bamburg JR
PLoS One. 2013 Dec 31;8(12):e83609. doi: 10.1371/journal.pone.0083609. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
50958ShuttleNSEA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter
50959ShuttleMCPA backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter
51279pmRFP-N1 human cofilin R21Qexpresses human cofilin R21Q mutant fused to mRFP in mammalian cells
245964CMV-huCof R21Q-mRFPExpression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods
245965huCof K22Q-mRFPExpression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Antibodies from Article