A genetically encoded reporter for real-time imaging of cofilin-actin rods in living neurons.
Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB, Bamburg JR
PLoS One. 2013 Dec 31;8(12):e83609. doi: 10.1371/journal.pone.0083609.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50958 | ShuttleNSE | A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a Neuron-specific enolase (NSE) promoter instead of the CMV promoter |
| 50959 | ShuttleMCP | A backbone for adenovirus creation by the AdEasy method, similar to ShuttleCMV (Addgene Plasmid # 16403), except it uses a mouse cofilin promoter (MCP) instead of the CMV promoter |
| 51279 | pmRFP-N1 human cofilin R21Q | expresses human cofilin R21Q mutant fused to mRFP in mammalian cells |
| 245964 | CMV-huCof R21Q-mRFP | Expression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods |
| 245965 | huCof K22Q-mRFP | Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods |