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Copper is required for oncogenic BRAF signalling and tumorigenesis.
Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ, Counter CM
Nature. 2014 Apr 9. doi: 10.1038/nature13180. PubMed Article

Plasmids from Article

ID Plasmid Purpose
53156pBABEbleo-Flag-BRAFV600EExpresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vector
53157pWZLblasti-CTR1Expresses human CTR1 from blasticidin resistance retroviral vector
53158pWZLblasti-CTR1M154AExpresses human CTR1 M154A from blasticidin resistance retroviral vector
53159pGEX6P1-HA-MEK1Expresses GST-HA tagged human MEK1 from bacterial expression vector
53161pWZLblasti-HA-MEK1Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector
53163pGEX6P1-HA-MEK1 CBMExpresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector
53164pGEX6P1-HA-MEK1 C121SExpresses GST-HA tagged human MEK1 C121S from bacterial expression vector
53165pWZLblasti-HA-MEK1 C121SExpresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector
53166pGEX6P1-HA-MEK5Expresses GST-HA tagged human MEK5 from bacterial expression vector
53167pWZLblasti-HA-MEK5Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector
53168pGEX6P1-HA-MEK5 CBM-EExpresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector
53169pWZLblasti-HA-MEK5 CBM-EExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector
53170pGEX4T3-HA-ERK2 K54RExpresses GST-HA tagged human ERK2 K54R from bacterial expression vector
53171pGEX6P1-HA-ERK5 K84RExpresses GST-HA tagged human ERK5 K84R from bacterial expression vector
53172pWZLblasti-HA-ERK2 R67SExpresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector
53173pWZLblasti-HA-ERK2 D321NExpresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector
53174pWZLblasti-HA-ERK2 GOFExpresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector
53175pWZLblasti-HA-ERK5Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector
53176pBABEpuro-HA-MEK1-MEK5DDExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from puromycin resistance retroviral vector
53177pWZLblasti-HA-MEK1-MEK5DDExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector
53178pBABEpuro-CMYC T58AExpresses mouse CMYC T58A from puromycin resistance retroviral vector
53179pSUPER-retro-puro-tetO-Mek1-shRNAExpresses shRNA against mouse Mek1 from puromycin resistance retroviral vector
53180pSUPER-retro-puro-tetO-CTR1-shRNAExpresses shRNA against human CTR1 from puromycin resistance retroviral vector
53195pBABEpuro-HA-MEK1Expresses HA tagged human MEK1 from puromycin resistance retroviral vector
53196pBABEpuro-HA-MEK1 CBMExpresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vector
53197pBABEpuro-HA-MEK1 C121SExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vector
53198pBABEpuro-HA-MEK5Expresses HA tagged human MEK5 from puromycin resistance retroviral vector
53199pBABEpuro-HA-MEK5 CBM-EExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vector
53200pGEX4T3-HA-ERK2 GOFExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector
53201pGEX4T3-HA-ERK2 R67SExpresses GST and HA tagged human ERK2 R67S from bacterial expression vector
53202pGEX4T3-HA-ERK2 D321NExpresses GST and HA tagged human ERK2 D321N from bacterial expression vector
53203pBABEpuro-HA-ERK2 GOFExpresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vector

Antibodies from Article