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Copper is required for oncogenic BRAF signalling and tumorigenesis.
Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S, Xiao K, Campbell SL, Thiele DJ, Counter CM
Nature. 2014 Apr 9. doi: 10.1038/nature13180.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53156pBABEbleo-Flag-BRAFV600EExpresses Flag tagged human oncogenic BRAFV600E from bleomycin resistance retroviral vector
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53157pWZLblasti-CTR1Expresses human CTR1 from blasticidin resistance retroviral vector
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53158pWZLblasti-CTR1M154AExpresses human CTR1 M154A from blasticidin resistance retroviral vector
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53159pGEX6P1-HA-MEK1Expresses GST-HA tagged human MEK1 from bacterial expression vector
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53161pWZLblasti-HA-MEK1Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector
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53163pGEX6P1-HA-MEK1 CBMExpresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vector
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53164pGEX6P1-HA-MEK1 C121SExpresses GST-HA tagged human MEK1 C121S from bacterial expression vector
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53165pWZLblasti-HA-MEK1 C121SExpresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector
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53166pGEX6P1-HA-MEK5Expresses GST-HA tagged human MEK5 from bacterial expression vector
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53167pWZLblasti-HA-MEK5Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector
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53168pGEX6P1-HA-MEK5 CBM-EExpresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vector
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53169pWZLblasti-HA-MEK5 CBM-EExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vector
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53170pGEX4T3-HA-ERK2 K54RExpresses GST-HA tagged human ERK2 K54R from bacterial expression vector
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53171pGEX6P1-HA-ERK5 K84RExpresses GST-HA tagged human ERK5 K84R from bacterial expression vector
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53172pWZLblasti-HA-ERK2 R67SExpresses HA tagged human ERK2 R67S from blasticidin resistance retroviral vector
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53173pWZLblasti-HA-ERK2 D321NExpresses HA tagged human ERK2 D321N from blasticidin resistance retroviral vector
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53174pWZLblasti-HA-ERK2 GOFExpresses HA tagged human ERK2 R67SD321N from blasticidin resistance retroviral vector
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53175pWZLblasti-HA-ERK5Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector
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53176pBABEpuro-HA-MEK1-MEK5DDExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from puromycin resistance retroviral vector
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53177pWZLblasti-HA-MEK1-MEK5DDExpresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector
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53178pBABEpuro-CMYC T58AExpresses mouse CMYC T58A from puromycin resistance retroviral vector
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53179pSUPER-retro-puro-tetO-Mek1-shRNAExpresses shRNA against mouse Mek1 from puromycin resistance retroviral vector
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53180pSUPER-retro-puro-tetO-CTR1-shRNAExpresses shRNA against human CTR1 from puromycin resistance retroviral vector
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53195pBABEpuro-HA-MEK1Expresses HA tagged human MEK1 from puromycin resistance retroviral vector
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53196pBABEpuro-HA-MEK1 CBMExpresses HA tagged human MEK1 M187A/H188A/M230A/H239A from puromycin resistance retroviral vector
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53197pBABEpuro-HA-MEK1 C121SExpresses HA tagged human MEK1 C121S from puromycin resistance retroviral vector
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53198pBABEpuro-HA-MEK5Expresses HA tagged human MEK5 from puromycin resistance retroviral vector
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53199pBABEpuro-HA-MEK5 CBM-EExpresses HA tagged human MEK5 L280A/H281A/M323A/Q332A from puromycin resistance retroviral vector
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53200pGEX4T3-HA-ERK2 GOFExpresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector
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53201pGEX4T3-HA-ERK2 R67SExpresses GST and HA tagged human ERK2 R67S from bacterial expression vector
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53202pGEX4T3-HA-ERK2 D321NExpresses GST and HA tagged human ERK2 D321N from bacterial expression vector
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53203pBABEpuro-HA-ERK2 GOFExpresses HA tagged human ERK2 R67S D321N from puromycin resistance retroviral vector
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