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Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells.
Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K
Nucleic Acids Res. 2014 Apr 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52522pRB14expresses a myc-tagged version of hCas9 in Drosophila
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52527pRB17serves as template for PCR-mediated fusion of U6-promoter with sgRNA
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52528pMH3serves as PCR-template to generate HR donors for C-terminal GFP-tag
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52529pMH4serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag
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52530pIW1serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag
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52531pMH5expresses FLP recombinase in Drosophila
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52532pKF251Double-GFP reporter for DNA cleavage
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52533pKF257expresses I-SceI nuclease in Drosophila
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52559pRB20serves as PCR-template to generate HR donors for C-terminal YFP-tag
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