Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy
Mazo-Vargas A, Park H, Aydin M, Buchler NE
Mol Biol Cell. 2014 Sep 17. pii: mbc.E14-07-1187.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 60114 | pNB774 | Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA) |
| 60115 | pNB775 | Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA) |
| 60117 | pNB776 | Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA) |
| 60119 | pNB687 | Yellow beetle luciferase |
| 60133 | pNB689 | Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter. |
| 60134 | pNB690 | Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter. |
| 60135 | pNB691 | Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter. |
| 60136 | pNB649 | Firefly luciferase (Promega) driven by MET17 promoter. |
| 60138 | pNB769 | Red click beetle luciferase from Promega driven by MET17 promoter. |
| 60139 | pNB768 | Green click beetle luciferase from Promega driven by MET17 promoter. |
| 60140 | pNB648 | NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter. |
| 60142 | pNB655 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter. |
| 60143 | pNB682 | Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter. |
| 60144 | pNB695 | Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA). |
| 60145 | pNB693 | Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA). |
| 60146 | pNB697 | Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA). |
| 60147 | pNB330 | Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS) |
| 60148 | pNB753 | Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter. |
| 60149 | pNB756 | Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter. |
| 60150 | pNB762 | Firefly luciferase (Promega) driven by LEU1 promoter. |
| 60151 | pNB786 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter. |
| 60152 | pNB787 | Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter. |
| 60153 | pNB777 | Red click beetle luciferase (Promega) driven by LEU1 promoter. |
| 60154 | pNB778 | Green click beetle luciferase (Promega) driven by LEU1 promoter. |
| 60155 | pNB752 | Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter. |
| 60156 | pNB761 | Firefly luciferase (Promega) driven by ADE17 promoter. |
| 60157 | pNB754 | Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter. |
| 60158 | pNB763 | Firefly luciferase (Promega) driven by LYS9 promoter. |
| 60159 | pNB767 | Firefly luciferase (Promega) driven by SIC1 promoter. |
| 60160 | pNB812 | Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter. |
| 60161 | pNB813 | Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter. |
| 60162 | pNB783 | Firefly luciferase (Promega) driven by RNR1 promoter. |
| 60163 | pNB784 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter. |
| 60164 | pNB785 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter. |
| 60165 | pNB788 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter. |
| 60166 | pNB789 | Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter. |