Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
Stolfi A, Gandhi S, Salek F, Christiaen L
Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 59986 | U6>sgRNA(F+E) | Empty vector for sgRNA cloning (F+E modified backbone) |
| 59987 | Eef1a-1955/-1>nls::Cas9::nls | Eef1a (EF1alpha) promoter driving nls::Cas9::nls |
| 59988 | Mesp-1916/-1>nls::Cas9::nls | Mesp driver driving nls::Cas9::nls |
| 59989 | Sox1/2/3-2373/+3>nls::Cas9::nls | Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls |
| 59990 | U6>Ebf.774(F+E) | U6 promoter driving Ebf.774 (F+E) sgRNA |
| 60006 | U6>Control(F+E) | U6 promoter driving control (F+E) sgRNA |