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Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.

Stolfi A, Gandhi S, Salek F, Christiaen L
Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
59986U6>sgRNA(F+E)Empty vector for sgRNA cloning (F+E modified backbone)
59987Eef1a-1955/-1>nls::Cas9::nlsEef1a (EF1alpha) promoter driving nls::Cas9::nls
59988Mesp-1916/-1>nls::Cas9::nlsMesp driver driving nls::Cas9::nls
59989Sox1/2/3-2373/+3>nls::Cas9::nlsSox1/2/3 (SoxB1) driver driving nls::Cas9::nls
59990U6>Ebf.774(F+E)U6 promoter driving Ebf.774 (F+E) sgRNA
60006U6>Control(F+E)U6 promoter driving control (F+E) sgRNA

Antibodies from Article