Jonathon Pines Lab:
Pines Lab plasmids
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61842 | pCeru-N1 cyclin A (1557) | mammalian expression of cyclin A with cerulean fusion |
| 61844 | pGEX 6P1 Cyclin A (555) | bacterial expression of GST cyclin A |
| 61845 | pGEX 6P1 CDK2 (552) | bacterial expression of GST CDK2 WT |
| 61847 | pcDNA5/FRT/TO SBBP-CDK1 WT (2007) | mammalian expression of CDK1 WT, used for Ips |
| 61848 | pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991) | for making stable RPE cells expressing WT cdc20 |
| 61850 | pIRES-mRFP-NLS-PCNA (2514) | cell cycle marker |
| 61852 | pCerulean-N1 Cyclin B1 (1147) | mammalian expression of Cyclin B1 fused to Cerulean |
| 61854 | pVenus-N1 (804) | empty backbone for tagging proteins at the C-terminus with Venus |