Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Jonathon Pines Lab: Pines Lab plasmids

Plasmids from Article

ID Plasmid Purpose
61842pCeru-N1 cyclin A (1557)mammalian expression of cyclin A with cerulean fusion
61844pGEX 6P1 Cyclin A (555)bacterial expression of GST cyclin A
61845pGEX 6P1 CDK2 (552)bacterial expression of GST CDK2 WT
61847pcDNA5/FRT/TO SBBP-CDK1 WT (2007)mammalian expression of CDK1 WT, used for Ips
61848pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)for making stable RPE cells expressing WT cdc20
61850pIRES-mRFP-NLS-PCNA (2514)cell cycle marker
61852pCerulean-N1 Cyclin B1 (1147)mammalian expression of Cyclin B1 fused to Cerulean
61854pVenus-N1 (804)empty backbone for tagging proteins at the C-terminus with Venus

Antibodies from Article