Escherichia coli auxotroph host strains for amino acid-selective isotope labeling of recombinant proteins.

Lin MT, Fukazawa R, Miyajima-Nakano Y, Matsushita S, Choi SK, Iwasaki T, Gennis RB
Methods Enzymol. 2015;565:45-66. doi: 10.1016/bs.mie.2015.05.012. Epub 2015 Jun 10. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
61953	YM138	C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.
61960	MS1	C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted
61961	RF11	C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted
61962	RF1	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted
62070	RF2	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted
62072	RF4	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.
62073	RF5	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted.
62074	RF6	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.
62075	RF8	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. asnA asnB genes deleted.
62076	RF10	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.
62077	RF12	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. trpA trpB genes deleted.
62078	RF13	BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.