CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells.
Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 10.1093/nar/gku1326. Epub 2014 Dec 18.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62715 | ptreCas9-mKate2ps-T1gRNA | Inducible; gRNA seq is ATGAGAATCAAGGCGGTCGA |
| 62716 | pCas9–mKate2ps–EgRNA | Empty; no U6 gRNA cassette |
| 62717 | pCas9–mKate2ps–T1gRNA | t1 gRNA (ATGAGAATCAAGGCGGTCGA) |
| 64955 | pCas9–mKate2ps–CgRNA | Control gRNA (GTCAAGGCACTCTTGCCTA) |