A Tn7-based broad-range bacterial cloning and expression system.

Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer RR, Schweizer HP
Nat Methods. 2005 Jun;2(6):443-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
63120	pUC18-mini-Tn7T-Gm-lacZ	mini-Tn7 lacZ transcriptional fusion vector
63121	pUC18T-mini-Tn7T-Gm	mini-Tn7 vector
64946	pFLP3	Flp recombinase expression vector
64948	pFCM1	source of CmR FRT cassette
64949	pFGM1	source of GmR FRT cassette
64950	pFKM1	source of KmR FRT cassette
64951	pFTC1	source of TcR FRT cassette
64952	pFTP1	source of TpR FRT cassette
64953	pUC18-mini-Tn7	mini-Tn7 base vector
64954	pUC18-mini-Tn7T	mini-Tn7 base vector with transcriptional terminator
64956	pUC18PV-mini-Tn7T	pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA
64957	pUC18T-mini-Tn7T	mini-Tn7 base vector with transcriptional terminator and oriT
64958	pUC18R6K-mini-Tn7T	mini-Tn7 base vector with transcriptional terminator
64961	pUC18-mini-Tn7T-Gm-GW	mini-Tn7 Gateway destination vector
64963	pUC18-mini-Tn7T-Gm-lux	mini-Tn7 luxCDABE transcriptional fusion vector
64965	pUC18-mini-Tn7T-LAC	mini-Tn7 vector with Ptac expression cassette
64966	pUC18T-mini-Tn7T-Gm-REP	mini-Tn7 vector with oriR6K, for attTn7 site determination
64967	pTNS1	Tn7 transposase expression
64968	pTNS2	Tn7 transposase expression
64969	pUC18R6KT-mini-Tn7T-Km	mini-Tn7 base vector with transcriptional terminator and oriT
65009	pFRT2	FRT cassette base vector