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CIDAR MoClo Parts Kit
(Kit # 1000000059 )

Depositing Labs:   Douglas Densmore

The Cross-disciplinary Integration of Design Automation Research lab (CIDAR) MoClo Library is collection of modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design and expression tuning in E. coli.

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$350 USD + shipping

Available to academics and nonprofits only.

Original Publication

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Iverson SV, Haddock TL, Beal J, Densmore DM. ACS Synth Biol. 2015 Nov 4. doi: 10.1002/bit.25814. PubMed .

Description

The CIDAR MoClo Library contains promoters, ribosomal binding sites, coding sequence, terminators, vectors, and a set of fluorescent control plasmids. Optimized protocols reduce reaction time and cost by >80% from previously published protocols.

Kit part diagram
Overview of CIDAR MoClo Parts Kit
Kit assembly workflow diagram
CIDAR MoClo Kit Workflow

Kit Documentation

Download a ZIP file containing the plasmid sequences in GenBank format:

Icon Genbank Files (228.3 KB)

Documents describing this kit:

Icon CIDAR MoClo summary (129.1 KB)

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059).”

For your Reference section:

CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. Iverson SV, Haddock TL, Beal J, Densmore DM. ACS Synth Biol. 2015 Nov 4. doi: 10.1002/bit.25814. PubMed .

CIDAR MoClo Parts Kit - #1000000059

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
A / 1 J23100_AB
Ampicillin
A / 2 J23100_EB
Ampicillin
A / 3 J23100_FB
Ampicillin
A / 4 J23100_GB
Ampicillin
A / 5 J23102_AB
Ampicillin
A / 6 J23102_EB
Ampicillin
A / 7 J23102_FB
Ampicillin
A / 8 J23102_GB
Ampicillin
A / 9 J23103_AB
Ampicillin
A / 10 J23103_EB
Ampicillin
A / 11 J23103_FB
Ampicillin
A / 12 J23103_GB
Ampicillin
B / 1 J23106_AB
Ampicillin
B / 2 J23106_EB
Ampicillin
B / 3 J23106_FB
Ampicillin
B / 4 J23106_GB
Ampicillin
B / 5 J23107_AB
Ampicillin
B / 6 J23107_EB
Ampicillin
B / 7 J23107_FB
Ampicillin
B / 8 J23107_GB
Ampicillin
B / 9 J23116_AB
Ampicillin
B / 10 J23116_EB
Ampicillin
B / 11 J23116_FB
Ampicillin
B / 12 J23116_GB
Ampicillin
C / 1 R0010 (pLacI)_AB
Ampicillin
C / 2 R0010 (pLacI)_EB
Ampicillin
C / 3 R0010 (pLacI)_FB
Ampicillin
C / 4 R0010 (pLacI)_GB
Ampicillin
C / 5 R0040 (pTet)_AB
Ampicillin
C / 6 R0040 (pTet)_EB
Ampicillin
C / 7 R0040 (pTet)_FB
Ampicillin
C / 8 R0040 (pTet)_GB
Ampicillin
C / 9 R0063 (pLuxR-pR)_AB
Ampicillin
C / 10 R0063 (pLuxR-pR)_EB
Ampicillin
C / 11 R0063 (pLuxR-pR)_FB
Ampicillin
C / 12 R0063 (pLuxR-pR)_GB
Ampicillin
D / 1 I13453 (pBAD)_AB
Ampicillin
D / 2 I13453 (pBAD)_EB
Ampicillin
D / 3 I13453 (pBAD)_FB
Ampicillin
D / 4 I13453 (pBAD)_GB
Ampicillin
D / 5 B0032m_BC
Ampicillin
D / 6 B0033m_BC
Ampicillin
D / 7 B0034m_BC
Ampicillin
D / 8 BCD12_BC
Ampicillin
D / 9 BCD2_BC
Ampicillin
D / 10 BCD8_BC
Ampicillin
D / 11 C0012m (lacI)_CD
Ampicillin
D / 12 C0040 (tetR)_CD
Ampicillin
E / 1 C0062 (luxR)_CD
Ampicillin
E / 2 C0080 (araC)_CD
Ampicillin
E / 3 cre_CD
Ampicillin
E / 4 E0030 (YFP)_CD
Ampicillin
E / 5 E0040m (GFP)_CD
Ampicillin
E / 6 E1010m (RFP)_CD
Ampicillin
E / 7 eBFP2 (eBFP2)_CD
Ampicillin
E / 8 B0015_DE
Ampicillin
E / 9 B0015_DF
Ampicillin
E / 10 B0015_DG
Ampicillin
E / 11 B0015_DH
Ampicillin
E / 12 DVA_AB
Ampicillin
F / 1 DVA_AE
Ampicillin
F / 2 DVA_AF
Ampicillin
F / 3 DVA_AG
Ampicillin
F / 4 DVA_AH
Ampicillin
F / 5 DVA_BC
Ampicillin
F / 6 DVA_CD
Ampicillin
F / 7 DVA_DE
Ampicillin
F / 8 DVA_DF
Ampicillin
F / 9 DVA_DG
Ampicillin
F / 10 DVA_DH
Ampicillin
F / 11 DVA_EB
Ampicillin
F / 12 DVA_EF
Ampicillin
G / 1 DVA_EG
Ampicillin
G / 2 DVA_EH
Ampicillin
G / 3 DVA_FB
Ampicillin
G / 4 DVA_GB
Ampicillin
G / 5 DVA
Ampicillin
G / 6 pJ02B2Gm_AE
Ampicillin
G / 7 pJ02B2Gm_EF
Ampicillin
G / 8 pJ02B2Rm_AE
Ampicillin
G / 9 pJ02B2Rm_EF
Ampicillin
G / 10 pJ02B2Gm:Rm_AF
Ampicillin
G / 11 pJ02B2Rm:Gm_AF
Ampicillin
G / 12 pJ02B2Gm_AE
Kanamycin
H / 1 pJ02B2Gm_EF
Kanamycin
H / 2 pJ02B2Rm_AE
Kanamycin
H / 3 pJ02B2Rm_EF
Kanamycin
H / 4 DVK_AE
Kanamycin
H / 5 DVK_AF
Kanamycin
H / 6 DVK_EF
Kanamycin
H / 7 DVK_FG
Kanamycin
H / 8 DVK_GH
Kanamycin
H / 9 DVK
Kanamycin
Data calculated @ 2017-03-25

Kit Plate Map - #1000000059

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