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Doudna Lab CRISPR Plasmids Available from Addgene

Assorted Cas9 nucleases from different bacteria as well as a mammalian codon-optimized Cas9 expression plasmid.

These plasmids are described in:

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. Science. 2012 Jun 28. PubMed PMID 22745249

Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA. Science. 2010 Sep 10;329(5997):1355-8. PubMed PMID 20829488

RNA-programmed genome editing in human cells. Jinek M, East A, Cheng A, Lin S, Ma E, Doudna JA. eLife. 2013 Jan 29;2:e00471. PubMed PMID 23386978

Individual plasmids can be ordered via the links below:

ID Plasmid
39312 pMJ806r: Streptococcus pyogenes Cas9.
39313 pMJ823: Listeria innocua Cas9.
39314 pMJ824: Streptococcus thermophilus Cas9.
39315 pMJ825: Streptococcus pyogenes Cas9 D10A mutation.
39316 pMJ826: Streptococcus pyogenes Cas9 H840A.
39317 pMJ839: Neisseria meningitidis Cas9.
39318 pMJ841: Streptococcus pyogenes Cas9 D10A, H840A.
41091 pHMGWA-Pa14Csy4: Pseudomonas aeruginosa Csy4 WT.
41092 pHMGWA-Pa14Csy4H29A: Pseudomonas aeruginosa Csy4 H29A.
42234 pMJ920: Human codon-optimized Cas9 expression plasmid.