MoClo Baculo Toolkit
(Kit #
1000000256
)
Depositing Lab: Chen Davidovich
This collection includes a set of plasmids designed to construct multigene expression vectors for the baculovirus system and yeast. Baculovirus expression is provided through compatibility with the Bac-to-Bac and biGBac systems. Yeast expression is provided through compatibility with the MoClo-YTK (Yeast Toolkit).
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Modular cloning of multigene vectors for the baculovirus system and yeast. Lai Z, Flanigan SF, Boudes M, Davidovich C. J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025. PubMed (Link opens in a new window) Article (Link opens in a new window)
Description
The MoClo Baculo Toolkit is a modular cloning system designed to simplify and speed up the generation of multigene expression constructs for the baculovirus expression system. The toolkit is based on Golden Gate Assembly, using Type IIS restriction enzymes (BsaI or BsmBI). Plasmids are typically assembled from multiple DNA parts in a one-pot, one-step reaction without PCR or Gibson assembly. The system supports flexible tagging of proteins dedicated for expression and purification, and provides compatibility with the biGBac Kit and MoClo-YTK.
The toolkit includes all the plasmids required for domesticating parts and using them to build baculovirus expression vectors of up to six genes each. The number of proteins that are expressed from a single vector can be increased up to 30 through compatibility with the biGBac Kit. Therefore, users who intend to use the MoClo Baculo Toolkit to build baculovirus expression vectors of seven or more proteins are advised to obtain the biGBac Kit in addition to the MoClo Baculo Toolkit. Users who wish to use their MoClo Baculo-compatible open reading frames and tags to build yeast expression vectors should obtain the MoClo-YTK in addition to the MoClo Baculo Toolkit.
- Figure 1: Simplified schematic of MoClo Baculo Toolkit workflow highlighting its compatibility with other systems.
Kit Documentation
A protocol can be found on the Davidovich Lab website (Link opens in a new window).
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The MoClo Baculo Toolkit was a gift from Chen Davidovich (Addgene kit #1000000256).”
For your Reference section:
Modular cloning of multigene vectors for the baculovirus system and yeast. Lai Z, Flanigan SF, Boudes M, Davidovich C. J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025. PubMed (Link opens in a new window) Article (Link opens in a new window)
MoClo Baculo toolkit - #1000000256
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol | |
| Chloramphenicol and Spectinomycin | |
| Ampicillin and Gentamicin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| A / 1 | pMB.003 | Chloramphenicol |
| A / 2 | pMB.005 | Chloramphenicol |
| A / 3 | pMB.006 | Chloramphenicol |
| A / 4 | pMB.007 | Chloramphenicol |
| A / 5 | pMB.008 | Chloramphenicol |
| A / 6 | pMY.001 | Chloramphenicol |
| A / 7 | pMY.002 | Chloramphenicol |
| A / 8 | pMY.003 | Chloramphenicol |
| A / 9 | pMY.004 | Chloramphenicol |
| A / 10 | pMY.005 | Chloramphenicol |
| A / 11 | pMY.006 | Chloramphenicol |
| A / 12 | pMY.007 | Chloramphenicol |
| B / 1 | pM.001 | Chloramphenicol |
| B / 2 | pM.002 | Chloramphenicol |
| B / 3 | pM.003 | Chloramphenicol |
| B / 4 | pM.004 | Chloramphenicol |
| B / 5 | pM.005 | Chloramphenicol |
| B / 6 | pM.006 | Chloramphenicol |
| B / 7 | pM.007 | Chloramphenicol |
| B / 8 | pM.008 | Chloramphenicol |
| B / 9 | pM.009 | Chloramphenicol |
| B / 10 | pM.010 | Chloramphenicol |
| B / 11 | pMB.004 | Chloramphenicol and Spectinomycin |
| B / 12 | pMB.001 | Ampicillin and Gentamicin |
| C / 1 | pMB.002 | Ampicillin and Gentamicin |
| C / 2 | pMB.BIG1a | Ampicillin and Gentamicin |
| C / 3 | pMB.BIG1b | Ampicillin and Gentamicin |
| C / 4 | pMB.BIG1c | Ampicillin and Gentamicin |
| C / 5 | pMB.BIG1d | Ampicillin and Gentamicin |
| C / 6 | pMB.BIG1e | Ampicillin and Gentamicin |
| C / 7 | pMB.Pos1 | Ampicillin and Gentamicin |
| C / 8 | pMB.Pos2 | Ampicillin and Gentamicin |
| C / 9 | pMB.Pos3 | Ampicillin and Gentamicin |
| C / 10 | pMB.Pos4 | Ampicillin and Gentamicin |
| C / 11 | pMB.Pos5 | Ampicillin and Gentamicin |
| C / 12 | pMB.Pos6 | Ampicillin and Gentamicin |
| D / 1 | pM.L3456 | Ampicillin |
| D / 2 | pM.L456 | Ampicillin |
| D / 3 | pM.L56 | Ampicillin |
| D / 4 | pM.L6 | Ampicillin |
| D / 5 | pM.000 | Chloramphenicol |