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Yarrowia lipolytica Golden Gate tool kit
(Kit # 1000000167 )

Depositing Lab:   Tristan Rossignol

A Golden Gate tool kit containing sixty-four standarized bricks for assembly of up to three transcription units for use in Yarrowia lipolytica

This kit will be sent as bacterial glycerol stocks in 96-well plate format.

Add to Cart
$375 USD + shipping

Available to academics and nonprofits only.

Original Publication

A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park Y-K, Soudier P, Kubiak M, Nicaud J-M, Rossignol T. Microb Biotechnol.2019 Nov;12(6):1249-1259. doi: 10.1111/1751-7915.13427. PubMed PMID: 31148366.

Description

The Yarrowia lipolytica Golden Gate tool kit contains a set of sixty-four standardized bricks that can be used for the one-step assembly of up to three transcription units. The toolbox includes six different selective markers (three auxotrophic markers, two antibiotic-resistance markers, and one metabolic marker), nine different promoters including inducible promoters for each transcription unit, up to six terminators, five pairs of sequences for genome integration and a set of genes coding for fluorescent proteins. The toolbox is made for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.

Schematic drawing of the Golden Gate assembly in Yarrowia lipolytica. Bottom indicates all the bricks available at each position in the three transcription units configuration with letters indicating the 4-nt overhang flanking each brick. Prom: promoter; Ter: terminator; InsertUp and InsertDown: sequences for insertion in the genome.

How to Cite this Kit

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

For your Materials and Methods section:

“The Yarrowia lipolytica Golden Gate tool kit was a gift from Tristan Rossignol (Addgene kit #1000000167).”

For your Reference section:

A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology. Larroude M, Park Y-K, Soudier P, Kubiak M, Nicaud, J-M, Rossignol T. Microb Biotechnol.2019 Nov;12(6):1249-1259. doi: 10.1111/1751-7915.13427. PubMed PMID: 31148366.

Yarrowia lipolytica Golden Gate tool kit - #1000000167

Resistance Color Key

Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.

Inventory

Searchable and sortable table of all plasmids in kit. The Well column lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.

Kit Plate Map

96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.

Resistance Color Key

Ampicillin
Kanamycin

Inventory

Well Plasmid Resistance
A / 1 GGE0029
Ampicillin
A / 2 GGE114
Ampicillin
A / 3 GGE083
Kanamycin
A / 4 GGE176
Kanamycin
A / 5 GGE105
Kanamycin
A / 6 GGE142
Kanamycin
A / 7 GGE367
Kanamycin
A / 8 GGE368
Kanamycin
A / 9 GGE085
Kanamycin
A / 10 GGE002
Kanamycin
A / 11 GGE001
Kanamycin
A / 12 GGE145
Kanamycin
B / 1 GGE146
Kanamycin
B / 2 GGE147
Kanamycin
B / 3 GGE104
Kanamycin
B / 4 GGE132
Kanamycin
B / 5 GGE250
Kanamycin
B / 6 GGE006
Kanamycin
B / 7 GGE005
Kanamycin
B / 8 GGE004
Kanamycin
B / 9 GGE150
Kanamycin
B / 10 GGE151
Kanamycin
B / 11 GGE152
Kanamycin
B / 12 GGE108
Kanamycin
C / 1 GGE139
Kanamycin
C / 2 GGE129
Kanamycin
C / 3 GGE009
Kanamycin
C / 4 GGE008
Kanamycin
C / 5 GGE007
Kanamycin
C / 6 GGE292
Kanamycin
C / 7 GGE294
Kanamycin
C / 8 GGE317
Kanamycin
C / 9 GGE273
Kanamycin
C / 10 GGE275
Kanamycin
C / 11 GGE259
Kanamycin
C / 12 GGE014
Kanamycin
D / 1 GGE177
Kanamycin
D / 2 GGE082
Kanamycin
D / 3 GGE025
Kanamycin
D / 4 GGE027
Kanamycin
D / 5 GGE015
Kanamycin
D / 6 GGE257
Kanamycin
D / 7 GGE256
Kanamycin
D / 8 GGE260
Kanamycin
D / 9 GGE080
Kanamycin
D / 10 GGE258
Kanamycin
D / 11 GGE286
Kanamycin
D / 12 GGE020
Kanamycin
E / 1 GGE021
Kanamycin
E / 2 GGE077
Kanamycin
E / 3 GGE270
Kanamycin
E / 4 GGE190
Kanamycin
E / 5 GGE070
Kanamycin
E / 6 GGE261
Kanamycin
E / 7 GGE067
Kanamycin
E / 8 GGE038
Kanamycin
E / 9 GGE091
Kanamycin
E / 10 GGE094
Kanamycin
E / 11 GGE255
Kanamycin
E / 12 GGE254
Kanamycin
F / 1 GGE253
Kanamycin
F / 2 GGE252
Kanamycin
F / 3 GGE207
Kanamycin
F / 4 GGE209
Kanamycin
Data calculated @ 2021-10-25

Kit Plate Map - #1000000167

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