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Human DECIPHER Lentiviral shRNA Library Mod 3
(Pooled Library #29589)

  • Purpose

    DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated human gene set.

    This module adds additional cell surface, extracellular, and DNA binding targets.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 29589 DECIPHER Lentiviral shRNA Library, Human Mod 3: Cell Surface, Extracellular, and DNA Binding Targets 1
Available to academic and nonprofits only.

Library Details

  • Species
    Human
  • Genes targeted
    4,922
  • shRNAs
    27,500

Library Shipping

Each library is delivered in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze/thaw cycles.

  • Volume
    ∼120 µL
  • Concentration
    1 µg/µL

Resource Information

Depositor Comments

Plasmids need to be grown in OmniMAX 2 T1R (Life Technologies) or SURE (Agilent) Recombination defective E. coli cells.

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. This module adds additional cell surface, extracellular, DNA binding targets, and other genes to the well-annotated signal transduction and disease-associated genes primarily targeted with the first two modules of the human shRNA library. Many of the genes targeted in Module 3 are conserved and appear integral to a variety of important biological process but are not as well characterized as the 10,000 genes targeted in the first two modules.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website for more information.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library, Human Mod 3: Cell Surface Targets was a gift from Alex Chenchik (Addgene #29589)