Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Mouse DECIPHER Lentiviral shRNA Libraries Mod 1 & 2
(Pooled Libraries #28287, #28288)

  • Purpose

    DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. They are made to cover the entire annotated mouse gene set.

    Module 1 is intended for the widest possible screen and targets annotated pathway genes.

    Module 2 covers disease-associated genes and drug targets that are not included in Module 1.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 28287 DECIPHER Lentiviral shRNA Library, Mouse Mod 1: Pathway Targets
Pooled Library 28288 DECIPHER Lentiviral shRNA Library, Mouse Mod 2: Disease Targets
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted

    Mouse Mod 1: Pathway Targets - 4625
    Mouse Mod 2: Disease Targets - 4520
  • shRNAs
    27,500

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼120µL
  • Concentration
    1µg/µL

Depositor Comments

DECIPHER libraries are barcoded lentiviral shRNA libraries optimized for RNAi Genetic Screens in pooled format. Mouse Module 2 can be used for the more comprehensive follow-up screens or simply be done in parallel. There are approximately 10,000 genes that have an Entrez/RefSeq ID and at least one interaction documented in PubMed and cover the majority of disease-associated genes and drug targets. About half of these are included in Module 1 while Module 2 covers the rest.

PLEASE NOTE: The DECIPHER shRNA are designed for target mRNA associated with the RefSeq#, not the HUGO gene symbol. We can't guarantee that the gene symbols indicated are accurate, as there may be synonyms, nomenclature updates/changes, or other discrepancies in naming that are beyond our control. Please see the HUGO Gene Nomenclature Committee website for more information.

How to cite this pooled library ( Back to top)

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    DECIPHER Lentiviral shRNA Library was a gift from Alex Chenchik & Gus Frangou (Addgene # )