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Purpose
This yeast two-hybrid library is a pair of double mutant libraries at 19 positions of the PYR1 gene. L007 (Library 183177) contains all proximal double mutations separated by fewer than eight amino acids. L008 (Library 183178) contains all distant double mutations separated by eight amino acids or greater.
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Vector Backbone
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 1000000181 | PYR1 Double Mutant Library | 1 | $ 540 | Add to Cart | |
Library Details
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SpeciesA. thaliana (mustard weed)
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Number of mutants42,743
Library Shipping
This library will be delivered as two pooled DNA libraries in microcentrifuge tubes on blue ice (tubes labeled L007 and L008). The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
Libraries can be analyzed using the Protein Analysis and Classifier Toolkit (PACT) available on GitHub (Link opens in a new window).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PYR1 Double Mutant Library was a gift from Timothy Whitehead (Addgene #1000000181) -
For your References section:
Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds. Beltrán J, Steiner PJ, Bedewitz M, Wei S, Peterson FC, Li Z, Hughes BE, Hartley Z, Robertson NR, Medina-Cucurella AV, Baumer ZT, Leonard AC, Park S-Y, Volkman BF, Nusinow DA, Zhong W, Wheeldon I, Cutler SR, Whitehead TA. Nat Biotechnol. 2022 Jun 20. doi: 10.1038/s41587-022-01364-5. PMID 35726092.