We narrowed to 1,855 results for: golden gate assembly

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Cultivarium MACKEREL Library

Pooled Library

#208054

Purpose

pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Use

Synthetic Biology

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only
pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly&activeSite

Plasmid

#248013

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&ActiveSite design)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly&activeSite

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pT7-module1

Plasmid

#222838

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module2

Plasmid

#222839

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module3

Plasmid

#222840

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module4

Plasmid

#222841

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module5

Plasmid

#222842

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module6

Plasmid

#222843

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 6)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module1*5

Plasmid

#222844

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1*5)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module2*5

Plasmid

#222845

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2*5)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDGG039

Plasmid

#231339

Purpose

Terminator; BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 T15m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDGG038

Plasmid

#231338

Purpose

Terminator; BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 T12m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNDGG037

Plasmid

#231337

Purpose

Terminator; BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module. Cloned via BpiI GG Assembly of PCR amplicon into POGG006, SpR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 T2m terminator with DF extension for CIDAR MoClo Golden Gate Assembly, DF module.

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module1

Plasmid

#222838

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module2

Plasmid

#222839

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module3

Plasmid

#222840

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module4

Plasmid

#222841

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module5

Plasmid

#222842

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-module6

Plasmid

#222843

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 6)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge