Addgene

We narrowed to 11,258 results for: Vars

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MAC_N_TLX2

Plasmid

#167806

Purpose

Expresses TLX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TLX2 (TLX2 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and eGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_4

Plasmid

#102966

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_4 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Use

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_3

Plasmid

#102965

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_3 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAINS4

Plasmid

#69456

Purpose

RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-IN S4 + SFGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

mutations for S4 variant (from wt)

Promoter

J23119 and J23119 (sigma 70)

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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